Synthesis, Characterisation and Biological Assessment of 2,4,6-Trisubstituted 1,3,5-Triazine Derivatives

Abstract

2,4,6-trichloro-1,3,5-triazine (Cyanuric Chloride) and 4-(4-aminophenyl)morpholin-3-one were converted into a new series of 2,4,6-trisubstituted 1,3,5-triazine derivatives by a regioselective reaction, which was followed by reflux with substituted thiophenole in the presence of a catalytic amount of triethyl amine. IR, 1H-NMR, mass, and elemental analysis were used to characterise each of the synthesised substances. All the synthesized compounds were tested for their in vitro antimicrobial activity against gram-positive strains, such as Staphylococcus aureus and Bacillus subtilis, as well as gram-negative strains, such as Escherichia coli and Pseudomonas aeruginosa, by using ampicillin, chloramphenicol and tetracycline as standard drugs. In-vitro antifungal activity was tested against three different fungal strains, such as Candida albicans, Aspergillus flavus and Aspergillus niger. Most of the compounds showed moderate to good activity. Among them, compound CML-1F is most active against bacterial species and compound CML-1H exhibited potent antifungal activity.