EVALUATION OF ANTIGLYCATION ACTIVITY AND GLYCATION REVERTING POTENTIAL OF CASSIA AURICULATA FLOWERS

Abstract

Cassia auriculata Linn. belongs to the Family Caesalpiniaceae and it is commonly called as Avaram in local name. It possess number of medicinal properties, especially it is used in the treatment of hyperglycemia. In the present study, in vitro antiglycation activity and glycation reverting potential of the flower extract (ethyl acetate) of Cassia auriculata was investigated. Antiglycation and Glycation reverting activity was studied using NBT and BSA glucose model assay respectively. Phytochemical analysis revealed that the flower extract contains bioactive constituents such as flavonoids, glycosides, phenols, tannins, quinone, coumarins, saponins and carbohydrates. The antiglycation activity of ethyl acetate extract was studied for a period of 3 weeks. Metformin was used as standard. There is a marked decrease in the reduction of NBT and prevention of glycation on 16th day. After 3 weeks of incubation the extract and drug showed maximum antiglycation activity of 82% and 90% respectively. The glycation reversing ability by extract and metformin increases with increase in concentration. The glycation reverting potential of metformin at concentrations of 100-500μg was 15% -75 % respectively and for the extracts (100-500 μg concentration) the glycation reverting potential was found be 4- 25%. This is the primary study aimed to reveal the glycation reverting ability of the ethyl acetate extract of Cassia auriculata. From this study, it can be concluded that the consumption of Cassia flowers as a decoction may play an important role in the controlling diabetes and its complications.