Synthesis, Characterization, Anti-Tumor and Anti-Microbial Activity of Fatty Acid Analog of 2, 6-Diisopropylphenol

Abstract

Derivative of 2, 6-diisopropylphenol (propofol) was prepared by coupling with 9-hydroxy-11-Z-octadecenoic acid (isolated from seed oil of Holarrhena antidysenterica) with the C1-α-hydroxy function of 2, 6-diisopropylphenol. The coupling reaction between fatty acid and propofol was carried out by dicyclohexylcarbodiimide in the presence of catalytic amount of dimethylaminopyridine to produce quantitative yield of the desired product. Spectroscopic studies confirmed the formation of the desired product. The compound was then investigated for its in-vitro anticancer activity against a panel of solid human tumor cell lines including HeLa, MCF-7 and HL-60(human leukemia) cells. Its cytotoxicity was also determined against non-cancerous mammalian cells (VERO cells) for comparison. The analog was cytotoxic against all cancer cell lines whereas no effect was observed against normal cells. The compound was also screened for its antimicrobial activity against E. coli, S. aureus and S. albus. The compound show good antimicrobial activity against E. coli and S. albus.